U2OS Cell stably expressing H1 Histamine Receptor, Calcium Biosensor & β-Arrestin Biosensor

Description Gentaur Cells are cell lines stably co-expressing tag-free GPCRs and two Gentaur Biosensors to monitor G-protein and β-arrestin modulation. Each vial of MPXGentaur H1 Histamine Receptor Cell Line contains U2OS cells stably expressing the following constructs: human H1 Histamine Receptor with no tag Calcium Gentaur Biosensor (Green Fluorescence) β-arrestin Gentaur Biosensor (Red Fluorescence) Gentaur H1 Histamine Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to H1 receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by ß-Arrestin. This leads to an increase of fluorescence intensity of both β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.