PCR Enhancing Reagent

The PCR Enhancing Reagent is supplied in 10 x conc. Simply add the reagent in PCR reactions in the 1x-3x final concentration. We recommend that for each target, a series of reactions are set up to titrate the concentration of the PCR Enhancing Reagent in 1x, 2x, and 3 x concentrations. The PCR Enhancing Reagent is also referred to as PCR Enhancer. The effective melting temperature of primers in a reaction containing this reagent is reduced. Therefore, a decrease in annealing temperature of PCR is often required. What is this for? High GC content DNA targets or DNA targets with secondary structures. A low-cost way to troubleshoot difficult PCR amplification. Also try our high Robustness PCR enzymes, including the PaCeR.