GB-Amp™ PowerGreen™ InFluor™ Green qPCR Mix

GB-Amp™ Power Green InFluor™ Green qPCR Mix For research use only Components Component P2101-01 P2101-02 P2101-03 2X Power Green qPCR Mix 1 ml 1 ml × 5 1 ml × 25 400 rxn/20μl reaction 2000 rxn/20μl reaction 10,000 rxn/20μl reaction Storage This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light. Description Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on. The PowerGreen™ InFluor™ HotStart Taq DNA Polymerase in the mix is chemically modified, and it can reduce non-specific amplification more effectively than the antibody-modified polymerase. At the same time, the mix contains aptamer-based inhibitors, which further reduce the generation of the primer dimer and secondary metabolites. With this dual blocking mechanism, when the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This dual hot-start technique brings greater efficiency and specificity to PCR. About InFluor™ Green-a nucleic acid (DNA and RNA) intercalating dye, which is fully compatible will all major real-time PCR (qPCR) systems, specifically, InFluor™ Green fluorescence can be read in the green channel of the real-time PCR thermal cyclers for quantitative PCR analyses. The InFluor™ does not inhibit PCR amplification. It supports melt curve analyses. Applications Gene expression analysis Low-copy gene detection Microarray validation Gene knockdown validation Features Compatible with all major real-time PCR systems, which accept No ROX (not requiring ROX reference dye), Low ROX or High ROX Exceptional specificity with dual hot-start mechanism Tight reproducibility in Ct values over a broad dynamic range Universal instrument compatibility Table of Instrument Guide Instruments ROX Cat. # ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700 High ROX P2101-01 P2101-02 P2101-03 ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 Low ROX P2101-01a P2101-02a P2101-03a Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler No ROX P2101-01b P2101-02b P2101-03b