FS Fast DNA Polymerase 2X Master Mix

The same high-performance Taq DNA polymerase, configured in a Master Mix format for your convenience. Fast Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be used in a variety of PCR. The FSTM PCR Buffer, designed for FSTM Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FSTM Taq DNA polymerase is more than twice the rate by regular Taq DNA polymerase, which shortens the amplification time at least by half , as fast as 5s/kb for simple sequence templates. Features Fast rate of elongation: elongation rate can reach to 3 kb/min, more than twice the rate by regular Taq DNA polymerase; Highly thermostable : have a half-life of over 40 min at 95°C incubation Generates 3'-dA overhangs PCR products. Applications Routine PCR PCR labeling PCR sequencing Generate PCR product for TA cloning Unit Definition One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate. Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests Functionally tested in amplification of a single-copy gene from human genomic DNA Store all components at –20°C Protocol for PCR All solutions should be thawed on ice, gently vortexed and briefly centrifuged. Add in a thin walled PCR tube on ice: