6x Gel Loading Buffer with Dyes

Quantity: 5 x 1 mL tubes (5 mL total) Gel Loading Buffer with Dye (6X) is a pre-mixed Loading Buffer with two tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping any ongoing reaction. Bromophenol blue and xylene cyanol are the standard tracking dye for electrophoresis. Contents Gel Loading Dye (6X): 60 mM Tris-HCL（pH 8.0） 0.06% Bromophenol Blue 0.03% Xylene Cyanol 60% Glycerol 60 mM EDTA Storage Store at RT Quality Assurance Statement Gel Loading Dye(6X) is assayed for contaminating endonuclease, exonuclease and RNase activity. Dye Migrate Rate (on a standard 1% TAE or TBE agarose gel) Xylene Cyanol TAE: 4160 bp TBE: 3030 bp Bromophenol Blue TAE: 370 bp TBE: 220 bp