CASP3 antibody

Volume: 100 µg Purification: protein A+G purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Mouse Clonality: monoclonal Clone ID: 2G7 Isotype: IgG1 Storage: 0.01MPBS pH 7.4,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3. Immunogen: caspase 3, apoptosis-related cysteine peptidase Synonyms: Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1 Observed MW: 32-35 kDa, 19 kDa, 17 kDa Uniprot ID: P42574 Reactivity: Human, Mouse, Rat Tested Application: ELISA, IHC, WB,IF Recommended dilution: WB: 1: 500-1: 2000; IHC: 1: 50-1: 300; IF: 1: 50-1: 200 Gene ID: 836 Research Area: Cancer, Metabolism, Neuroscience