C-FOS antibody

Volume: 100 µg Purification: Immunogen affinity purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: Isotype: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: c-FOS, also named as Proto-oncogene c-Fos and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP(basic-leucine zipper) domain and belongs to the bZIP family. FOS is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, FOS undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the FOS protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the FOS protein becomes nuclear. The calculated molecular weight of FOS is 40 kDa, but Phosphorylated FOS protein is about 60-65 kDa. Immunogen: Proto oncogene c Fos Synonyms: AP 1, C FOS, Cellular oncogene fos, c-FOS, FOS, G0S7, Proto oncogene c Fos Observed MW: 65 kDa Uniprot ID: P01100 Reactivity: Human, Mouse, Rat Tested Application: ELISA, IF, IHC, WB Recommended dilution: WB: 1: 500-1: 2000; IHC: 1: 200-1: 800; IF: 1: 50-1: 500 Gene ID: 2353 Research Area: Signal Transduction, Immunology, Cancer