DDX21 antibody

Volume: 100 µg Purification: Immunogen affinity purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: Isotype: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase(Pol) I and II: promotes ribosomal RNA(rRNA) processing and transcription from polymerase II(Pol II)(PubMed: 25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs(PubMed: 25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes(PubMed: 25470060, PubMed: 25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes(PubMed: 25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'(PubMed: 11823437, PubMed: 25260534). Can unwind double-stranded RNA(helicase) and can fold or introduce a secondary structure to a single-stranded RNA(foldase)(PubMed: 9461305). Involved in rRNA processing(PubMed: 14559904, PubMed: 18180292). Immunogen: DEAD(Asp-Glu-Ala-Asp) box polypeptide 21 Synonyms: Observed MW: 87 kDa Uniprot ID: Q9NR30 Reactivity: Human, Mouse, Rat Tested Application: ELISA, WB, IHC, IF, IP Recommended dilution: WB: 1: 500-1: 2000; IP: 1: 200-1: 1000; IHC: 1: 20-1: 200; IF: 1: 20-1: 200 Gene ID: 9188 Research Area: Metabolism