CTCF antibody

Volume: 100 µg Purification: Immunogen affinity purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: Isotype: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20? for 12 months (Avoid repeated freeze / thaw cycles.) Background: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Immunogen: CCCTC-binding factor (zinc finger protein) Synonyms: Observed MW: 135 kDa Uniprot ID: P49711 Reactivity: Human, Mouse, Rat Tested Application: ELISA, WB, IHC, IP Recommended dilution: WB: 1: 1000 - 1: 2000; IHC: 1: 50 - 1: 200; ChIP : 1: 50 - 1: 200 Gene ID: 10664 Research Area: Epigenetics, Cell Division and Proliferation, Metabolism