CLP1 antibody

Volume: 100 µg Purification: Immunogen affinity purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: Isotype: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA(dsRNA), single-stranded RNA(ssRNA), double-stranded DNA(dsDNA) and double-stranded DNA: RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA: RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA(PubMed: 24766809, PubMed: 24766810). Its role in tRNA splicing and maturation is required for cerebellar development(PubMed: 24766809, PubMed: 24766810). Component of the pre-mRNA cleavage complex II(CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs(siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex(RISC). However, endogenous siRNAs and microRNAs(miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. Immunogen: CLP1, cleavage and polyadenylation factor I subunit, homolog(S. cerevisiae) Synonyms: HEAB Observed MW: 48 kDa Uniprot ID: Q92989 Reactivity: Human, Mouse Tested Application: ELISA, WB Recommended dilution: WB: 1: 500-1: 2000 Gene ID: 10978 Research Area: Metabolism