CHCHD4 antibody

Volume: 100 µg Purification: Immunogen affinity purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: Isotype: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17 or MICU1(PubMed: 16185709, PubMed: 26387864, PubMed: 19182799, PubMed: 21059946, PubMed: 23186364). Required for the import and folding of small cysteine-containing proteins(small Tim) in the mitochondrial intermembrane space(IMS)(PubMed: 16185709, PubMed: 19182799, PubMed: 21059946). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria(PubMed: 16185709, PubMed: 19182799, PubMed: 21059946). The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS(PubMed: 16185709, PubMed: 19182799, PubMed: 21059946). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system(PubMed: 23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake(PubMed: 26387864). Immunogen: coiled-coil-helix-coiled-coil-helix domain containing 4 Synonyms: MIA40 Observed MW: 22-25 kDa Uniprot ID: Q8N4Q1 Reactivity: Human, Mouse, Rat Tested Application: ELISA, IHC, IF, WB, IP Recommended dilution: WB: 1: 500-1: 2000; IP: 1: 200-1: 1000; IHC: 1: 20-1: 200; IF: 1: 20-1: 200 Gene ID: 131474 Research Area: Signal Transduction, Metabolism