BRCC3 antibody

Volume: 100 µg Purification: Immunogen affinity purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: Isotype: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains(PubMed: 19214193, PubMed: 20656690, PubMed: 24075985, PubMed: 26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks(DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks(DSBs)(PubMed: 20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates(PubMed: 20656690, PubMed: 24075985, PubMed: 26344097, PubMed: 26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex(CSN), via the interaction of the BRISC complex with the CSN complex(PubMed: 19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1(PubMed: 26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression(PubMed: 24075985, PubMed: 26344097). Down-regulates the response to bacterial lipopolysaccharide(LPS) via its role in IFNAR1 deubiquitination(PubMed: 24075985). Immunogen: BRCA1/BRCA2-containing complex, subunit 3 Synonyms: BRCC36, C6.1A, CXorf53 Observed MW: 65-70kd Uniprot ID: P46736 Reactivity: Human, Mouse, Rat, Monkey Tested Application: ELISA, WB, IHC Recommended dilution: WB: 1: 500-1: 2000; IHC: 1: 20-1: 200 Gene ID: 79184 Research Area: Signal Transduction, Cancer