ALKBH1 antibody

Volume: 100 µg Purification: Immunogen affinity purified Form: liquid Purity: 95% as determined by SDS-PAGE Host: Rabbit Clonality: polyclonal Clone ID: Isotype: IgG Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: Dioxygenase that acts as a DNA demethylase(PubMed: 18603530). Requires molecular oxygen, alpha-ketoglutarate and iron(PubMed: 18603530). Specifically demethylates DNA methylated on the 6th position of adenine(N(6)-methyladenosine) DNA. N(6)-methyladenosine(m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing(By similarity). Also able to repair alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation, but with low activity(PubMed: 18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites(PubMed: 19959401). DNA lyase activity does not require alpha-ketoglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product(PubMed: 19959401, PubMed: 23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation(By similarity). Immunogen: alkB, alkylation repair homolog 1(E. coli) Synonyms: ABH, ABH1, alkB, ALKBH, ALKBH1, DNA lyase ABH1, hABH Observed MW: 44 kDa Uniprot ID: Q13686 Reactivity: Human, Mouse, Rat Tested Application: ELISA, WB, IHC Recommended dilution: WB: 1: 200-1: 2000; IHC: 1: 20-1: 200 Gene ID: 238 Research Area: Metabolism