Acetylated tubulin(Lys40) antibody

Volume: 100 µg Purification: Protein A+G purification Form: liquid Purity: 95% as determined by SDS-PAGE Host: Mouse Clonality: monoclonal Clone ID: 6A10 Isotype: IgG1 Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background: Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. Immunogen: tubulin, alpha 1a Synonyms: Acetylated alpha tubulin, Acetylated tubulin, acetylated-a-tubulin, acetyl-a-tubulin, alpha tubulin, Alpha tubulin 3, tubulin,TUBA1A Observed MW: 52 kDa Uniprot ID: Q71U36 Reactivity: Human, Mouse, Rat, Dog Tested Application: ELISA, WB, IHC, IF Recommended dilution: WB: 1: 500-1: 5000; IF: 1: 20-1: 200; IHC: 1: 20-1: 200 Gene ID: 7846 Research Area: Neuroscience, Cell Division and Proliferation, Metabolism