DAPK3 (Ab-265) Antibody

Size : 100 µL Clone Number: Aliases: DAP kinase 3 antibody; DAP like kinase antibody; DAP-like kinase antibody; Dapk 3 antibody; DAPK3 antibody; DAPK3_HUMAN antibody; Death associated kinase 3 antibody; Death associated protein kinase 3 antibody; Death-associated protein kinase 3 antibody; Dlk antibody; EC 2.7.11.1 antibody; FLJ36473 antibody; MYPT1 kinase antibody; ZIP antibody; ZIP kinase antibody; ZIP kinase isoform antibody; ZIP-kinase antibody; ZIPK antibody; zipper-interacting protein kinase antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: O43293 Immunogen: Synthesized non-phosphopeptide derived from Human DAPK3 around the phosphorylation site of threonine 265 (R-M-T(p)-I-A). Host: Rabbit Reactivity: Human, Mouse Tested Applications: ELISA, WB, IHC; WB: 1: 500-1: 3000, IHC: 1: 50-1: 100 Background: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca2+ and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, its association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Kawai T., Mol. Cell. Biol. 18: 1642-1651(1998). Murata-Hori M., FEBS Lett. 451: 81-84(1999). The MGC Project Team; Genome Res. 14: 2121-2127(2004). Clonality: Polyclonal Isotype: IgG Purification Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjugate: Non-conjugated Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: DAPK3 Research Areas: Cancer;Cell biology;Signal transduction