CDK4 Antibody

Size : 100 µL Clone Number: Aliases: Cdk 4 antibody; cdk4 antibody; CDK4 protein antibody; CDK4_HUMAN antibody; Cell division kinase 4 antibody; Cell division protein kinase 4 antibody; CMM 3 antibody; CMM3 antibody; Crk3 antibody; Cyclin dependent kinase 4 antibody; Cyclin-dependent kinase 4 antibody; Melanoma cutaneous malignant 3 antibody; MGC14458 antibody; p34 cdk4 antibody; PSK J3 antibody; PSK-J3 antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: P11802 Immunogen: Peptide sequence around aa.295~299(L-H-K-D-E)derived from Human CDK4. Host: Rabbit Reactivity: Human Tested Applications: ELISA, WB, IHC; WB: 1: 500-1: 1000, IHC: 1: 50-1: 200 Background: Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle . Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. Sherr, C.J. (1996) Science 274, 1672-1677. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925. Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9. Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Conjugate: Non-conjugated Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: CDK4 Research Areas: Isotype/Loading Controls;Epigenetics and Nuclear Signaling;Cancer;Cell biology