CUL2 Antibody

Size : 100 µL Clone Number: Aliases: Cul2 antibody; CUL 2 antibody; CUL-2 antibody; CUL2 antibody; CUL2_HUMAN antibody; cullin 2 antibody; cullin homolog 2 antibody; Cullin-2 antibody; MGC131970 antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: Q13617 Immunogen: Synthesized peptide derived from Human Cullin 2. Host: Rabbit Reactivity: Human, Mouse Tested Applications: ELISA, WB, IHC, IF; WB: 1: 500-1: 3000, IHC: 1: 50-1: 100, IF: 1: 100-1: 500 Background: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). Seiji Shiraishi, J. Biol. Chem., Mar 2007; 282: 9017 - 9028. Isabelle Groulx, J. Biol. Chem., Mar 2000; 275: 8991. Dimitris Liakopoulos, PNAS, May 1999; 96: 5510. Kyungwon Huh, AACR Meeting Abstracts, Apr 2006; 2006: 1326. Clonality: Polyclonal Isotype: IgG Purification Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjugate: Non-conjugated Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: CUL2 Research Areas: Cancer;Cell biology