BUB1 Antibody

Size : 100 µL Clone Number: Aliases: Bub1 antibody; BUB1 budding uninhibited by benzimidazoles 1 homolog antibody; BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody; BUB1 mitotic checkpoint serine/threonine kinase antibody; BUB1; S. cerevisiae; homolog of antibody; BUB1_HUMAN antibody; BUB1A antibody; BUB1L antibody; Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody; Budding uninhibited by benzimidazoles 1 homolog antibody; Budding uninhibited by benzimidazoles 1; S. cerevisiae; homolog of antibody; hBUB1 antibody; Homolog of mitotic checkpoint gene BUB1 antibody; Mitotic checkpoint gene BUB1 antibody; Mitotic checkpoint serine/threonine protein kinase BUB1 antibody; Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody; Mitotic spindle checkpoint kinase antibody; Putative serine/threonine protein kinase antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: O43683 Immunogen: Recombinant protein of Human BUB1 Host: Rabbit Reactivity: Human, Mouse, Rat Tested Applications: ELISA, WB, IHC; WB: 1: 500-1: 2000, IHC: 1: 50-1: 200 Background: The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions. During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase. Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers. Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome. Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility. Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores, and is required for functional microtubule-kinetochore interactions. Clonality: Polyclonal Isotype: IgG Purification Method: Affinity purification Conjugate: Non-conjugated Buffer: Store at -20°C or -80°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: BUB1 Research Areas: Cancer;Cell biology