Acetyl-P53 (Lys120) Antibody
Volume: 100 µL
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
GeneName: TP53
GeneID: 7157
Uniprot: P04637
Specificity: Acetyl-P53 (Lys120) Antibody detects endogenous levels of P53 only when acetylated at Lys120.
Immunogen: A synthesized peptide derived from human P53 around the acetylation site of Lys120.
Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity: Human
Prediction:
Application: ELISA(peptide)
Dilution: ELISA(peptide) 1: 20000-1: 40000
Conjugate: Unconjugated
RRID: AB_2847059
An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide: AF3745-BP
Des# Blocking Peptide: Acetyl-P53 (Lys120) Blocking peptide
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Internal Reference:
AF3745