Cat Interferon ? (IFN-?) ELISA Kit

Reactivity: Cat (Felis catus,Feline) UniProt: P46402 Abbreviation: IFN-? Aliases: IFG; IFI; IFNG Application: ELISA Range: 6.25-400 pg/mL Sensitivity: 3.5 pg/mL Intra-AssayCV: ?6.8% Inter-AssayCV: ?9.7% Recovery: 1.01 Sample Type: Serum, Plasma, Other biological fluids Detection Method: Sandwich Analysis Method: Quantitative Test principle: This assay employs a two-site sandwich ELISA to quantitate IFN-? in samples. An antibody specific for IFN-? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIFN-? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-? is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-? bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).