Bovine MAP kinase-activated protein kinase 3 (MAPKAPK3) ELISA Kit

Reactivity: Bovine (Bos taurus; Cattle) UniProt: Q3SYZ2 Abbreviation: MAPKAPK3 Aliases: 3PK; MAPKAP3; MAPKAP kinase 3|OTTHUMP00000210806 Application: ELISA Range: Request Information Sensitivity: Request Information Intra-AssayCV: ?4.5% Inter-AssayCV: ?7.5% Recovery: 0.91 Sample Type: Serum, Plasma, Other biological fluids Detection Method: Sandwich Analysis Method: Quantitative Test principle: This assay employs a two-site sandwich ELISA to quantitate MAPKAPK3 in samples. An antibody specific for MAPKAPK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAPK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAPK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAPK3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview: MAPKAPK3 functions as a mitogen-activated protein kinase (MAP kinase)-activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).