In the world of molecular biology, efficient and high-quality RNA synthesis is critical for various research and industrial applications. Yeasen Bio offers an impressive array of products that cater to RNA synthesis needs, particularly with T7 RNA polymerase and RNase R. These tools are essential for researchers working in RNA structure and function studies, probe hybridization, RNA interference (RNAi), and more.
T7 RNA Polymerase: Cleascript™ for High Yield and Precision
Yeasen Bio's T7 RNA polymerase is derived from bacteriophage T7, modified and optimized for recombinant expression in E. coli. This advanced version reduces the production of double-stranded RNA (dsRNA), which can be problematic in certain applications. Using double-stranded DNA templates with the T7 promoter sequence, T7 RNA polymerase synthesizes complementary single-stranded RNA downstream of the promoter region.
Key Features of T7 RNA Polymerase:
- Enhanced RNA Integrity: Yeasen’s T7 mutant variant shows RNA integrity up to 89.9%, outperforming competitors.
- Low dsRNA Content: The T7 mutant version significantly reduces dsRNA formation, essential for applications that require high-quality single-stranded RNA.
- Flexible Template Compatibility: Suitable for double-stranded linear blunt-end or 5' protruding end DNA, allowing for versatility in template usage.
The Cleascript™ T7 RNA Polymerase is available in multiple specifications (10KU, 100KU, 250KU), providing flexibility based on research requirements.
Comparing T7 RNA Polymerase Variants: Wild-Type vs. Mutant
Yeasen Bio’s comparison between the wild-type (WT) and mutant T7 RNA polymerase variants shows significant improvements in RNA integrity and reduction of dsRNA content with the mutant version. This distinction is valuable for researchers needing higher precision in their RNA synthesis processes.
T7 High Yield RNA Synthesis Kit
For researchers focused on high-output applications, the T7 High Yield RNA Synthesis Kit is a powerful solution. This kit is designed to maximize transcription efficiency, producing 100-200 µg of RNA from just 1 µg of template. The synthesized RNA is suitable for applications such as RNA structure studies, RNAase protection assays, and microinjection.
Components of the Kit:
The kit includes T7 RNA polymerase, a 10X transcription buffer, and nucleotides (ATP/GTP/CTP/UTP). The flexibility of this kit allows for RNA labeling by incorporating modified nucleotides, which is beneficial for downstream detection.
RNase R for Selective RNA Digestion
RNase R, a magnesium-dependent exonuclease, is essential for selectively digesting linear RNA while preserving circular RNA species. This makes it invaluable for researchers studying RNA splicing events, especially the identification of circular RNA (circRNA) structures. RNase R is produced according to GMP standards, ensuring high quality and reproducibility in experimental results.
Applications of RNase R:
- Splicing Event Analysis: By digesting linear RNA, RNase R helps identify circular RNA species, enabling deeper insights into RNA splicing events.
- RNA Sequencing Preparation: Ideal for removing unwanted RNA forms before sequencing, RNase R enhances sequencing clarity and depth.
Yeasen’s RNase R is available in multiple formats (500 U, 5000 U, 20 KU, 200 KU), catering to various experimental scales.
Product Performance: Yield and Purity Comparisons
Yeasen Bio also provides compelling performance data showing their products' efficacy in RNA synthesis. A comparison of Yeasen’s T7 RNA polymerase with other companies shows higher yield and purity across different RNA lengths. Notably, Yeasen achieves superior results in yield (mg/mL) for 100nt, 200nt, and even up to 4000nt RNA, making it a reliable choice for high-throughput and high-fidelity RNA synthesis.
Purification Efficiency:
The data also highlight Yeasen’s advantages in RNA purification, whether through column purification or LiCl precipitation, showcasing the product’s adaptability for various downstream processes.
Summary
Yeasen Bio’s suite of RNA synthesis products, including T7 RNA polymerase, the High Yield RNA Synthesis Kit, and RNase R, offers researchers highly efficient, scalable, and customizable tools. These products are optimized for high yield, low dsRNA production, and excellent RNA integrity, meeting the rigorous demands of modern molecular biology research.
For researchers working in RNA synthesis, Yeasen Bio’s solutions provide unmatched quality and reliability, ensuring better experimental outcomes and enabling deeper insights into RNA functions and structures.